Antagonistic interactions among bacteria inhabiting pineapple. Sn comprehensive clinical medicine, volume 1, issue 3. Scientific information about tatumella species is scarce, as are confirmed human infections. Population dynamics were explored in coinoculation in vitro and in planta with strain uaps07070 and t. One yeast isolate, citeromyces matritensis, had a proteolytic activity of 2. Tatumella ptyseos, an unrevealed causative agent of pink disease in pineapple. Diversity of endophytic enterobacteria associated vith different host. This bacterial disease is caused by various brucella species, which mainly infect goats, sheep, cattle, swine, and dogs2. Globally, less than 10 bloodisolated human case reports were found after a pubmed search keywords. Isolation, identification and antimicrobial susceptibility pattern of. Comparative wholegenome sequence analysis and multilocus sequence analysis mlsa between tatumella ptyseos atcc 33301t and clinical isolate nml 063099t suggested that the clinical isolate was closely related to, but distinct from, the species t.
Spontaneous organic cocoa bean box fermentations in brazil. Pdf tatumella ptyseos is the type species of the tatumella genus enterobacteriaceae. Shifts in the midgutpyloric microbiota composition within a. Tatumella ptyseos is a rodshaped, gramnegative facultative anaerobic bacteria belonging to the.
It does not survive well when stored in conventional ways, and its flagella are positioned as either polar, subpolar, or lateral. Isolation, identification and antimicrobial susceptibility pattern of tatumella ptyseos strains isolated from powdered infant formula milk consumed in neonatal intensive care unit. Phylogeny and identification of pantoea species associated. Tatumella ptyseos is a rodshaped, gramnegative, facultative, and anaerobic bacteria categorized in the enterobacteriaceae family. Unlike other enterobacteria, tatumella has polar flagella. The culture collections hold cell cultures, bacteria, fungi and virus strains from worldwide sources. Five species, erwinia cypripedii, ewingella americana, rahnella aquatilis, serratia marcescens at 20, and tatumella ptyseos produced 2,5diketogluconate from glucose without a requirement for pyrroloquinoline quinone pqq. Isolation, identification and antimicrobial susceptibility pattern of tatumella ptyseos strains isolated from powdered infant formula milk consumed in neonatal. Affected fruit acquire a brownish pigment after pasteurization and can contaminate nonaffected fruit before they are released to the consumer. Spontaneous organic cocoa bean box fermentations in brazil are characterized by a restricted species diversity of lactic acid bacteria and acetic acid bacteria. It is a summary two hundred beef samples randomly collected from bodija. Our scientists ensure that the identification of the cultures is correct and they remain unchanged from when they are first deposited with the collection.
To date, only two cases have been reported in children. Positive isolates were further examined in liquid culture for their protease activities by hydrolysis of casein at different ph values 5. Comparative wholegenome sequence analysis and multilocus sequence analysis mlsa between tatumella ptyseos atcc 33301t and clinical isolate. Loop is the open research network that increases the discoverability and impact of researchers and their work.
It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. Pdf tatumella ptyseos is a rodshaped, gramnegative, facultative, and anaerobic bacteria categorized in the enterobacteriaceae. This strain was isolated from drosophila suzukii larvae as part of a larger project to study the microbiota of d. In affected fruit populations around 10 6 cfug of fresh tissue were recovered. Tatumella ptyseos is a rodshaped, gramnegative facultative anaerobic bacteria belonging to the enterobacteriaceae family and assigned to the family of erwiniaceae in 2016. Citeseerx document details isaac councill, lee giles, pradeep teregowda. Enterobacteriaceae brenner, 1992 in bergeys manual. Pink disease is a major problem in the pineapple canning industry. Isolation, identification and antimicrobial susceptibility. Tatumella, causal agent of pink disease, and strain uaps07070 was selected for further assays. Total avulsion and transection of the esophagus at the esophagogastric junction are very rare after blunt trauma, and their management is challenging. Pdf isolation, identification and antimicrobial susceptibility pattern. Proteolytic activities of bacteria, yeasts and filamentous.
On this basis, molecular identification procedures for the tatumella isolates associated with pink disease were implemented. Tatumella ptyseos, an unrevealed causative agent of pink. Other species were divided into two or more classes particularly escherichia coli, which. Case report in august 2017, an 81yearold woman was admitted at the emergency department of the chuv in lausanne with acute. The initial emergency operation should aim to save the life and native esophagus. The current study aimed to investigate the isolation, identification and antimicrobial susceptibility pattern of t.
Pink disease is asymptomatic in the field and is characterized by the production of dark discoloration amberreddish brown and its initial effects are not recognized until the fruits are cored and canned. Unusual stability of manganese superoxide dismutase from a. Pdf tatumella ptyseos, an unrevealed causative agent of. Total esophageal avulsion at the esophagogastric junction. In the present study, bacterial species associated with sound and botrytisinfected grapes of two cultivars originating from the same vineyard were assessed.
Grape bacterial microbiota plays central roles in the quality of grapes and wine, yet its diversity remains poorly described. Diagnosis of cattle brucellosis by pcr and serological. This strain was isolated from drosophila suzukii larvae as part of a. Introduction brucellosis is a zoonotic disease, and an important public health problem in many parts of the world, especially in the middle east region1. Taxonomic diversity of the dglucose oxidation pathway in the. Polyphasic taxonomic analysis was performed on a clinical isolate nml 063099t from a cystic fibrosis patient, including wholegenome sequencing, proteomics, phenotypic testing, electron microscopy, chemotaxonomy and a clinical investigation. We use cookies to offer you a better experience, personalize content, tailor advertising, provide social media features, and better understand the use of our services. Gramstainnegative, oxidasenegative, facultatively anaerobic, motile, rodshaped, nonpigmented bacterial strains n6po6t, n8po1 and n8pi1 were isolated from the mirid bug capsodes infuscatus captured on asphodelus aestivus plants. Pulmonary infection of tatumella ptyseos developed on the background of pulmonary tuberculosis. International journal of systematic and evolutionary microbiology 60.
We provide comprehensive resources for bacterial identification, genomics and microbiome. Here, we present the case of a boy with this injury. American microbiologist enteric section, centers for disease control and prevention, atlanta, ga, usa taxon names authored list may be incomplete. Investigation of prevalence of hbv dna in the individuals of negative hbs ag in yazd, iran. Loop enables you to stay uptodate with the latest discoveries and news, connect with researchers and form new collaborations. Furthermore, the japanese species are closely related to tatumella ptyseos, strains of which originate from human clinical specimens.
Tatumella ptyseos which was first characterized by hollis 1981 derived its name from harvey tatum a cdc microbiologist and ptyseos, epithet of sputum hollis et al. This fermentative gramnegative rod has only rarely been reported as a cause of human infections. Otu9 comprised of isolates obtained from citrus plants and identified according. Help pages, faqs, uniprotkb manual, documents, news archive and biocuration projects. The 16s rrna gene sequences of the strains shared 94.
Dnadna hybridization and phenotypic tests confirmed the observed phylogenetic distance of p. Tatumella ptyseos dsm 5000 tatumella ptyseos lmg 7888 tatumella ptyseos str. Tatumella ptyseos is an oxidasenegative, fermentative, gramnegative rod which grows on mac conkey agar. Tatumella named to honor harvey tatum, an american bacteriologist who made many contributions to our understanding of the classification and identification of fermentative and nonfermentative bacteria of medical importance. A genomic dna of 1416 bp containing an open reading frame encoding a manganese superoxide dismutase mnsod from tatumella ptyseos ct was cloned. Sequence analysis of this new gene revealed that it translates 205 amino acid residues. All cations in bergeys manual ofdeterminative bacte incubations were at 36 1c unless otherwise noted. Over 250 isolates showed a very high similarity in their phenotypic and genotypic traits. Strains of both tatumella ptyseos and tatumella morbirosei are reported to cause pink disease in pineapple. Proteobacteria gammaproteobacteria enterobacteriales enterobacteriaceae tatumella. Transfer of pantoea citrea, pantoea punctata and pantoea.
Isolation of tatumella ptyseos from beef in ibadan, nigeria. These analyses showed that the five most abundant bacteria identified were. A few biochemical characteristics distinguish tatumella ptyseos from the other members. Pdf tatumella ptyseos causing severe human infection. Classification of enterobacteriaceae by minimization of stochastic. Comparative wholegenome sequence analysis and multilocus sequence analysis mlsa between tatumella ptyseos atcc 33301t and. Bacterial species associated with sound and botrytis. Dna dna hybridization and phenotypic tests confirmed the observed phylogenetic distance of p. Isolates were identified by pcrdenaturing gradient gel electrophoresis pcrdgge and sequence analysis of. It is a rare foodborne opportunistic pathogen which causes neonatal sepsis, bacteremia, and urinary tract infections. Tatumella ptyseos is the type species of the tatumella genus enterobacteriaceae. Four hundred and eighty isolates of pineapple were tested for antagonistic activity vs. This is the first report of human infection in switzerland.
Request pdf on dec 12, 2018, baudouin bourlond and others published tatumella ptyseos causing human sepsis. Mar 01, 2005 read unusual stability of manganese superoxide dismutase from a new species, tatumella ptyseos ct. A total of 68% of these isolates were from sputum specimens. Therefore, we performed an indepth blast search of 1,000 random illumina sequences. In the concatenated tree and all individual gene trees, the japanese species clustered with t.
This bacteria, previously known as the cdc group ef9, was first described by hollis et al. Assembling a taxonomically balanced genomescale reconstruction of the evolutionary history of the enterobacteriaceae. At 24 h, the colonies on blood agar are nonhaemolytic, 0. Since the first description, six different species from the tatumella genus were described. Download fulltext pdf download fulltext pdf download fulltext pdf. Pineapple is the second most important tropical fruit in international trade and mexico ranks ninth in world production. Transfer of pantoea citrea, pantoea punctata and pantoea terrea to the genus tatumella emend. Sn comprehensive clinical medicine, volume 1, issue 3 springer. Us84267b2 methods and probes for detecting a vancomycin. In the last few years, pantoea citrea has been described as the causative agent of pink disease.